Volumetric morphometry reveals mitotic spindle width as the best predictor of spindle scaling

We also introduce Spindle3D, an open-source plug-in that allows for the quantitative, unbiased, and automated analysis of 3D fluorescent data of spindles and chromatin.

Spindle3D: https://sites.imagej.net/Spindle3D

with Christian Tischer @ EMBL

Congrats Abin! And thanks for past and ongoing teamwork @GuckLab

Thanks to Carolyn Moores @BirkbeckUoL for being our guest in today's lab meeting! Microtubules rock!

Thanks Flo for updating us on #denoising #CARE #Noise2Void #DivNoising @FijiSc

Spindles on the second day. Wow, congrats!

In Vitro Reconstitution and Imaging of Microtubule Dynamics by Fluorescence and Label-free Microscopy. 

Congrats to Will and thanks to the Schäffer lab for a great collaboration!

https://star-protocols.cell.com/protocols/283

Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts

Congrats Seb, Abin & Will!
https://star-protocols.cell.com/protocols/232

It's fun to freeze some brain!

Will and Abin's paper on how differences in frog microtubule dynamics result in different spindle lengths - now online at Current Biology! #OpenAccess

Adrian's paper on the effects of proliferation status and cell cycle phase on the responses of single cells to chemotherapy now online at Mol Biol Cell.

Coronavirus lockdown - 1st virtual lab meeting.

Stay strong & healthy everyone!

Congrats to Will and Abin, their paper has been accepted for publication in Current Biology. Well done!
Stay tuned, paper should be online and in press soon!

Please, let's remember Suzanne as a wonderful person and a remarkable scientist:
"Suzanne Eaton: 1959–2019" in Nature Cell Biology 21, 1053–1054 (2019).
"Suzanne Eaton (1959–2019)" in Cell 179-1, 18-20 (2019).
"Tribute to Suzanne Eaton, from her lab members" in Nature 572, 178 (2019).

"Suzanne Eaton (1959-2019)" in Development 2019 146: dev185538.
"Suzanne Eaton (1959–2019): A pioneer in quantitative tissue morphogenesis" in J Cell Biol (2019) 218 (9): 2819–2821.

Ella is joining us from France for 3 months! Have a great summer in Berlin, Ella!

11.000 marched for Science in Berlin. Thank you all. Science matters!

Simone is invited by the Simons Foundation to talk about ‘Biological Active Matter: Nongeneric Problems, Nongeneric Methods’ in the Flatiron building in New York!

Helena Jambor teaching us about science data visualization. Thanks Helena!

We were awarded a BSIO grant together with Clemens Schmitt, who works on cancer genetics and cellular stress responses!

Welcome Tobias!
Tobias is a joint master student with the Ewers lab!

Moving to The Marine Biological Laboratory in Woods Hole for the summer. From June - August our address is

Reber lab

Rowe 308, 7 Mbl St

Woods Hole

MA 02543

USA

Simone is invited to talk about "Using optics to measure subcellular dynamics and mechanics" at the at the BIMSB.

Imaging the Neuronal Microtubule Cytoskeleton

Microtubules are essential cytoskeletal components of neurons. They are fundamental to many neuronal processes, such as long-distance transport, but they also establish and maintain neuronal morphology. We are interested in dynamic as well as structural parameters of the microtubule cytoskeleton, such as microtubule number, length, distribution, orientation, and bundling throughout neuronal differentiation. The aim of this imaging-based project is to resolve the spatial organization of the microtubule cytoskeleton throughout neuronal differentiation with nanometer precision. 

Individuals experienced / interested in neurobiology, biophysics, biochemistry or bioengineering are invited to apply. Please send a CV, a letter of motivation, and names of up to 3 references via email to helge.ewers@fu-berlin.de and simone.reber@iri-lifesciences.de.  

Keywords: Microtubule cytoskeleton, neuronal differentiation, superresolution microscopy, nanobodies, quantitative imaging.

Thanks to the group of Michalis Averof, Patricia and all other PhD students for inviting Simone to their Student-Invited Seminar Series at the Institute of Functional Genomics of Lyon (IGFL).

Adrian came from the Department of Systems Biology from Havard Medical School and will continue his work on DNA repair.

Great job Seb!

Thanks Alex for a wonderful seminar on ‘Regulation of microtubule instability towards ensuring genome stability’.

Tony Hyman visits the lab and speaks about "Phase Separation in Cytoplasm: Implications for Polarity and Neurodegeneration". It was a pleasure!

Well done Abin!

Thanks Ivo for coming all the way from Lisbon and giving a great lecture on ‘Drosophila early embryogenesis under a new light’.

Entertaining Abendkolloquium by WIKO fellow Tim Caro.

Image courtesy: Wissenschaftskolleg zu Berlin.

Welcome Varun Kapoor, who is a joint postdoc with the Preibisch lab. Varun will work on the development of image analysis software to characterize microtubule dynamics.

Thanks to Dan  for coming all the way from Boston to talk about 'Physical Aspects of Spindle Assembly'.

Here’s a photo from the lab’s Christmas market outing. Wishing everyone wonderful holidays and a happy new year!

Image analysis workshop with Jan Brocher from BioVoxxel – great success!

We warmly welcome Leonie Ringrose as a new IRI professor! Great guest lectures by Susan Gasser (FMI, Basel), Martin Howard (JIC, Norwich), and Marc Rehmsmeier (HU Berlin) including a very British reception with scones, cucumber sandwiches, and Gin & Tonic. Way to go Leonie!

Volumetric morphometry reveals mitotic spindle width as the best predictor of spindle scaling

We also introduce Spindle3D, an open-source plug-in that allows for the quantitative, unbiased, and automated analysis of 3D fluorescent data of spindles and chromatin.

Spindle3D: https://sites.imagej.net/Spindle3D

with Christian Tischer @ EMBL

Congrats Abin! And thanks for past and ongoing teamwork @GuckLab

Thanks to Carolyn Moores @BirkbeckUoL for being our guest in today's lab meeting! Microtubules rock!

Thanks Flo for updating us on #denoising #CARE #Noise2Void #DivNoising @FijiSc

Spindles on the second day. Wow, congrats!

In Vitro Reconstitution and Imaging of Microtubule Dynamics by Fluorescence and Label-free Microscopy. 

Congrats to Will and thanks to the Schäffer lab for a great collaboration!

https://star-protocols.cell.com/protocols/283

Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts

Congrats Seb, Abin & Will!
https://star-protocols.cell.com/protocols/232

It's fun to freeze some brain!

Will and Abin's paper on how differences in frog microtubule dynamics result in different spindle lengths - now online at Current Biology! #OpenAccess

Adrian's paper on the effects of proliferation status and cell cycle phase on the responses of single cells to chemotherapy now online at Mol Biol Cell.

Coronavirus lockdown - 1st virtual lab meeting.

Stay strong & healthy everyone!

Congrats to Will and Abin, their paper has been accepted for publication in Current Biology. Well done!
Stay tuned, paper should be online and in press soon!

Please, let's remember Suzanne as a wonderful person and a remarkable scientist:
"Suzanne Eaton: 1959–2019" in Nature Cell Biology 21, 1053–1054 (2019).
"Suzanne Eaton (1959–2019)" in Cell 179-1, 18-20 (2019).
"Tribute to Suzanne Eaton, from her lab members" in Nature 572, 178 (2019).

"Suzanne Eaton (1959-2019)" in Development 2019 146: dev185538.
"Suzanne Eaton (1959–2019): A pioneer in quantitative tissue morphogenesis" in J Cell Biol (2019) 218 (9): 2819–2821.

Ella is joining us from France for 3 months! Have a great summer in Berlin, Ella!

11.000 marched for Science in Berlin. Thank you all. Science matters!

Simone is invited by the Simons Foundation to talk about ‘Biological Active Matter: Nongeneric Problems, Nongeneric Methods’ in the Flatiron building in New York!

Helena Jambor teaching us about science data visualization. Thanks Helena!

We were awarded a BSIO grant together with Clemens Schmitt, who works on cancer genetics and cellular stress responses!

Welcome Tobias!
Tobias is a joint master student with the Ewers lab!

Moving to The Marine Biological Laboratory in Woods Hole for the summer. From June - August our address is

Reber lab

Rowe 308, 7 Mbl St

Woods Hole

MA 02543

USA

Simone is invited to talk about "Using optics to measure subcellular dynamics and mechanics" at the at the BIMSB.

Imaging the Neuronal Microtubule Cytoskeleton

Microtubules are essential cytoskeletal components of neurons. They are fundamental to many neuronal processes, such as long-distance transport, but they also establish and maintain neuronal morphology. We are interested in dynamic as well as structural parameters of the microtubule cytoskeleton, such as microtubule number, length, distribution, orientation, and bundling throughout neuronal differentiation. The aim of this imaging-based project is to resolve the spatial organization of the microtubule cytoskeleton throughout neuronal differentiation with nanometer precision. 

Individuals experienced / interested in neurobiology, biophysics, biochemistry or bioengineering are invited to apply. Please send a CV, a letter of motivation, and names of up to 3 references via email to helge.ewers@fu-berlin.de and simone.reber@iri-lifesciences.de.  

Keywords: Microtubule cytoskeleton, neuronal differentiation, superresolution microscopy, nanobodies, quantitative imaging.

Thanks to the group of Michalis Averof, Patricia and all other PhD students for inviting Simone to their Student-Invited Seminar Series at the Institute of Functional Genomics of Lyon (IGFL).

Adrian came from the Department of Systems Biology from Havard Medical School and will continue his work on DNA repair.

Great job Seb!

Thanks Alex for a wonderful seminar on ‘Regulation of microtubule instability towards ensuring genome stability’.

Tony Hyman visits the lab and speaks about "Phase Separation in Cytoplasm: Implications for Polarity and Neurodegeneration". It was a pleasure!

Well done Abin!

Thanks Ivo for coming all the way from Lisbon and giving a great lecture on ‘Drosophila early embryogenesis under a new light’.

Entertaining Abendkolloquium by WIKO fellow Tim Caro.

Image courtesy: Wissenschaftskolleg zu Berlin.

Welcome Varun Kapoor, who is a joint postdoc with the Preibisch lab. Varun will work on the development of image analysis software to characterize microtubule dynamics.

Thanks to Dan  for coming all the way from Boston to talk about 'Physical Aspects of Spindle Assembly'.

Here’s a photo from the lab’s Christmas market outing. Wishing everyone wonderful holidays and a happy new year!

Image analysis workshop with Jan Brocher from BioVoxxel – great success!

We warmly welcome Leonie Ringrose as a new IRI professor! Great guest lectures by Susan Gasser (FMI, Basel), Martin Howard (JIC, Norwich), and Marc Rehmsmeier (HU Berlin) including a very British reception with scones, cucumber sandwiches, and Gin & Tonic. Way to go Leonie!